Feb 24, 2014 · Thus, the hold-up volume (time) is equal to the retention volume (time) of an unretained compound. The hold-up volume (time) includes any volumes contributed by the sample injector, the detector, and connectors. tM = VM Fc In gas. this term is also called the gas hold-up volume (time). The corrected gas hold-up volume ( Vo M) is the gas hold-up