Jan 20, 2014 · Hydrophilic polypropylene membrane GHP-200, 0.2 μm were from Pall Corporation (Mississauga, ON, Canada); micro cover glasses 25 × 25 mm 2 and plain glass microscope slides 75 × 25 mm 2 were from Aijiren Tech Scientific (Ottawa, ON, Canada). All solutions were prepared with 18.2 MΩ·cm water from Barnstead NanoPure Diamond (18 MΩ) water