GH Polypro (GHP) Membrane Disc Filters All purpose, universal membrane with maximum chemical compatibility for both aqueous solutions and aggressive solvents Specifications Filter Media Hydrophilic polypropylene Pore Size 0.2 and 0.45 µm Typical Thickness 0.2 µm: 101 µm (4.0 mils) 0.45 µm: 114 µm (4.5 mils) Typical Water Flow Rate mL/min
Acrodisc® Syringe Filters. Pall Laboratory. of filter media: hydrophilic nylon membrane for excellent chemical compatibility with esters, bases, and alcohols, without pre-wetting; GHP (hydrophilic polypropylene) membrane for low protein binding; and hydrophilic PVDF (polyvinylidine fluoride) membrane for use in aqueous and non-aggressive,.
Millex-FG, 0.20 µm, hydrophobic PTFE, 25 mm, A 25 mm diameter sterile syringe filter with a 0.2 µm pore size hydrophobic PTFE membrane, ethylene oxide sterilized. Millex-SV 5.0 µm, A 25 mm diameter sterile syringe filter with a 5 µm pore size hydrophilic PVDF membrane. Comes in a pack of 50.
10 Pack Syringe Filter Sterile PES Hydrophilic Filtration 0.22um Pore Size, 33mm Membrane Diameter Sterile PES Membrane Individually Packed by Membrane Solutions 4.5 out of 5 stars 377 #1 Best Seller in Syringe Lab Filters
of bacteria such as finished, potable water, use 100 mL of sample. Use less sample for non-potable water or water that contains more bacteria. When the approximate bacteria level is unknown, analyze three different sample volumes. Use the results from the sample volume that shows approximately 20 to 200 colonies for each membrane filter.
6X means a 6X dilution is required, so say for 10ul, it will be 10ul/6 = 1.7ul of your sample buffer to load to 8.3ul of your samples. -jiajia1987- so for example, if I have a stock that has a concentration of 10 micrograms/microliter and I want a concentration per lane of 100 microgram/microliter
28143-014PK 165.34 USD. CA28140-037 28143-014 28140-160 28140-037. Membrane Discs with GHP Membrane, Pall Laboratory. Pall GHP Polypropylene membrane will be discontinued effective October 31st, 2019. This discontinuation affects devices including Membrane Discs, Syringe Filters, Filter Plates, and Centrifugal Devices.
30ul total volume). Use a boiling water bath to boil protein samples for 2-3 min then cool on ice for 5 min and do a quick centrifuge to spin down sample because buffer “soapy.” sample buffer is 950ul Laemmli dye + 50ul beta-mercaptoethanol. 2x sample buffer made to match 1:1 the volume of the protein and dilution amount.
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Nest syringe filters have pore size either 0.22μm or 0.45μm, and diameter 13mm or 25mm, with membrane materials either with PES or PVDF membranes. For sample volumes of 2mL to 100mL Retention volume <115µL Maximum operating temperature: 100°C autoclave at 125°C
The 25 mm Acrodisc is recommended for sample volumes from 5 to 100 mL. The 13 mm size is recommended for samples under 10 mL in volume. The 4 mm size is for samples under 2 mL. PP housing with Luer fittings. GHP - hydrophilic PP membrane suitable for both aqueous and organic samples; GXF - multi-layered glass fiber prefilter
You add 5mL of water, so your final volume is 15mL. Then the dilution factor is final volume over initial volume, in this case: 15mL/10mL = 1.5. In your example, the final volume is the total volume you have after adding the liquid. If you already had sample in the cuvet, then the final volume is the volume you had plus the volume you added.
Ensuring the correct filtration membrane has been selected from different types of filter materials is often overlooked in routine laboratory sample filtration. Here, we explore different filtration properties and how to take a preventative—rather than reactive—approach, minimizing time spent troubleshooting and maximizing efficiency.
Calculate volume for lets say 30ug (30/conc lets say 3ug), so volume required is 10ul, add 1/3rd of 4X dye, i.e 3.33ul and add this to 10ul sample, load the entire 13.33 ul sample. Thats it
HTD96b is our standard model. The HTD96c version was designed to reduce the time required to reach equilibrium by decreasing the diffusion path length and increasing the dialysis membrane surface area to sample volume ratio. The reduced well volume design minimizes assay sample volumes (25-75ul) to reduce reagent costs.
