Nov 02, 2012 · GHP membranes filters were obtained from Pall Gelman with different porosities, 0.10 μm, 0.22 μm and 0.45 μm. Anion exchange column CarboPac PA-1 (4 mm × 250 mm, Analytical) and size exclusion column OHPak SB 806 M HQ (8 mm × 300 mm, particle size 13 μm, maximum pore size 15,000 Å, exclusion limit 20,000,000 based on pullulans) were