The GHP hydrophilic polypropylene (GH Polypro) membrane is ideal for maximum chemical compatibility, fast flow rates, low protein binding, and low levels of UV-absorbing extractables. The glass fiber membrane allows for quick and easy filtration for difficult-to-filter aqueous solutions.

All-purpose, universal hydrophilic polypropylene membrane with maximum chemical compatibility when filtering both aqueous solutions and aggressive solvents. Low protein binding of GH Polypro membrane provides high recovery of critical proteinaceous samples. HPLC certified to assure that the filter will not add artifacts to your analysis.

and low non-specific protein and nucleic acid binding. Add versatility – Available in various membrane types including low-binding Bio-Inert ® (modified nylon), Supor ® (polyethersulfone), and GHP (polypropylene) membranes, as well as Omega™ (modified polyethersulfone) ultrafiltration membrane in a variety of MWCOs.

are especially recommended for applications requiring low protein binding, such as filtering culture media containing sera. However, both cellulose acetate and cellulose nitrate membranes are naturally hydrophobic and have small amounts (less than 1%) of non-toxic wetting agents added during manufacture to ensure proper wetting of the membrane.

Jul 11, 2008 · To determine the tolerance of each membrane to chemicals, white polycarbonate, MF-Millipore, cellulose acetate, hydrophilic polypropylene and hydrophilic PTFE membrane filters that had low auto-fluorescence were mounted in chambers (37 mm monitor unit; Advantec Co. Ltd., Tokyo, Japan).

A possible candidate was the 17 kDa membrane protein CcoH because we had recently shown that CcoH is in close contact with CcoP . CcoH was originally considered to function as an assembly factor for cbb 3 Cox [ 31 ], but unexpectedly, CcoH forms a stable complex with the fully assembled cbb 3 Cox, and thus behaves more like a subunit [ 33 ].

SDH is a protein complex of the inner mitochondrial membrane composed of four nuclear-encoded subunits, two of which are integral membrane proteins of the inner mitochondrial membrane (Rutter et al., 2010). SDH is both an enzyme of the tricarboxylic acid cycle and a component of the electron transport chain.

Apr 01, 2005 · Binding assays were carried out at 25°C for 30 min in a final volume of 25 μl, containing 25 mmol l –1 Tris-HCl, pH 7.5, 125 I-apoA-I and 0.6 mg ml –1 of membrane protein. After incubation, the reaction mixture was cooled on ice and layered onto 400 μl of a sucrose cushion containing 25 mmol l –1 Tris-HCl, pH 7.5, and 250 mmol l –1

Chemically resistant membrane with low protein binding. • Hydrophilic membrane for aqueous or organic sample matrixes • Use with protein or peptide-based assays The hydrophilic polypropylene membrane is easily wetted with water and does not require pre-treatment with alcohols. Compatible with biological samples. Do not use with strong

In Gram-negative bacteria, MacA is believed to couple ATP hydrolysis to transport of substrates across the outer membrane through a TolC-like channel. In this study, we report a real-time analysis of concurrent ATP hydrolysis and assembly of MacAB-TolC complex. MacB binds nucleotides with a low millimolar affinity and fast on- and off-rates.

Herpesviruses require membrane-associated glycoproteins gB, gH, and gL for entry into host cells. Epstein-Barr virus (EBV) gp42 is a unique protein also required for viral entry into B cells. Key interactions between EBV gp42 and the EBV gH/gL complex were investigated to further elucidate their rol

In this study, we quantified the adsorption of immunoglobulin G (IgG) protein onto several polyelectrolyte-modified sintered porous polyethylene (PPE) membranes. The polymer surfaces had both cationic and anionic charges obtained via the adsorption of polyethylenimine (PEI) and polyacrylic acid (PAA

The remaining aqueous phase was extracted by diethyl ether (3 × 500 µl) and the extracts were combined in a 2 ml glass vial, and dried prior to reconstitution with 50 µl of methanol. To conduct the LC/MS analysis, these samples were also filtered through a 0.45 µm GH Polypro (GHP, hydrophilic polypropylene) membrane NanoSep MF centrifuge tube.

The membrane is peripherally bonded to an annular polypropylene ring (except the 13 mm diameter disc) for ease of handling and is suitable for both vacuum and pressure filtration Anopore is available in 3 nominal pore sizes: 0.02 µm, 0.1 µm, and 0.2 µm, and in 3 diameters: 13 mm, 25 mm, and 47 mm

PES (Polyethersulfone) Membranes. These hydrophilic, low protein binding, PES membrane filters are ideal for tissue culture media sterilization, life science and microbiology fluid applications, clinical, and general filtration. Microporous PES membranes are produced from a high-temperature, acid and base resistant, polyethersulfone polymer in