Nov 09, 2017 · The running buffer was 50 mmol L-1 sodium phosphate buffer with 150 mmol L-1 NaCl (Sigma-Aldrich) at a pH of 7.0, prepared with 0.22 µm filtration (GSWP04700, Merck KGaA). 10 µL of a 0.2 µm vacuum-filtered sample (0.2 µm GHP AcroPrep™ 96 filter plate; Pall Life Sciences, Ann Arbor, MI, USA) was applied to a TSKgel® G3000SWXL HPLC Column